Phic31 attp site

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August undefined, 2024

WebPhiC31 Integrase Expression Plasmid Put the integrase into your PhiC31 and PinPoint genome editing projects with this vector that expresses PhiC31 Integrase for one-step single-copy transgenesis Non-viral transgene delivery Single-copy integration Preferential integration at active sites Delivery of inserts with no size constraints WebOct 28, 2024 · This example illustrates a method of screening a variant of phiC31 integrase that recognizes a mutant attP site. The target mutant attP site was selected based on the following criteria: 1) it has a GC content of 20-60%; 2) it is symmetrically similar to the wildtype phiC31 attP site; 3) it is unique in the human genome; 4) it is intergenic; 5 ...

A powerful method combining homologous recombination and site ... - PNAS

WebApr 1, 2004 · Abstract. The φC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition sites. Here we show that this site-specific integration system also functions efficiently in Drosophila melanogaster in cultured cells and in … WebMay 7, 2013 · When phiC31-mediated site-specific integration occurs at the chromosomal attP site of the host strain, the whole sequence of the donor plasmid containing the attB site is inserted into the genome (Fig. 3a). To determine how such a reaction occurred in the established lines, we first performed inverse PCR and determined the flanking sequences. simple baked beef and pasta casserole

(PDF) Identification of a Specific Pseudo attP Site for Phage phiC3 …

WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … WebphiC31 attP Alt name phiC31 phage attachment site Species Streptomyces phage phiC31 Insert Size (bp) 221 Cloning Information Cloning method Restriction Enzyme 5′ … WebIt is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the … simple baked beans recipes to make

Creation of Engineered Human Embryonic Stem Cell Lines Using phiC31 …

Targeted integration into pseudo attP sites of CHO cells using …

WebJan 1, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in... Webthrough recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, and ... attB site phiC31 integrase donor vector formats SV40 promoter SV40 poly-A CMV TK poly-A pUC ori pFC-CMV-MCS-SV40-Kan/Neo Single Promoter phiC31 Vector Cat ... simple baked beans with ground beefWebJun 12, 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP. raveready reviews

"WebWe injected the donor plasmid together with the phiC31 integrase mRNA into the embryos of the host strain and obtained positive lines. Structural analysis of the lines showed that site-specific integration occurred by recombination between the genomic attP site and the attB site of the donor plasmid. " - Phic31 attp site

Phic31 attp site

(PDF) Identification of a Specific Pseudo attP Site for Phage phiC3 …

WebApr 8, 2024 · For example, we have developed the phiC31 integrase system for use in mammalian cells. This phage integrase pairs two short recognition sites, called attB and attP, and catalyzes recombination that results in chromosomal integration. This figure shows one approach we have developed with phiC31 integrase. WebOct 8, 2024 · An engineered phiC31 "Disintegrase" able to make an attP site in Drosophila out of an attR-attL pair is described. This was used to generate attP sites at genomic …

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WebOct 8, 2024 · One is a mutagenized phiC31 Integrase protein capable of mediating recombination between attR and attL sites to yield an attP site. The other is a P-element … WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide …

WebAug 20, 2024 · Finally, the candidate Phic31 pseudo attP site located in chromosome 6 was identified as an optimal integration site compared to the pseudo attP site of chromosome … WebJul 14, 2024 · The ΦC31 recombinase system is an important member of the S-rec family [ 2 ]. Both HK and ΦC31 systems comprise attB / attP attachment sites that serve as points of recombination, and the recombinases that catalyze recombination. In each family, DNA exchange requires host-encoded proteins for recombination that differ between systems.

WebIsolated from the bacteriophage PhiC31, the PhiC31 integrase (frequently also written as: ΦC31 integrase) encodes a serine-type recombinase that mediates the sequence-specific … WebEnter the email address you signed up with and we'll email you a reset link.

WebSequences of PhiC31 and BxB1 attachment sites For each recombinase, the attB and attP attachment sites share a common central dinucleotide sequence (in red) that is used for the strand exchange...

WebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … simple baked boneless chicken breastsWebJun 17, 2010 · Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination. While the phiC31 system has been recently applied in mouse and human cell lines and in Drosophila, it has not been demonstrated whether it can also catalyze efficient DNA recombination in zebrafish. raver cruz brotherWebDec 1, 2024 · Three novel PhiC31 pseudo-attP sites were identified on chromosomes 1, 3 and 6, and their genomic features were analyzed. The characterized platform cell lines are stable, and because of single-copy, site-specific R4 recognition attP site, the cell lines could be retargeted for recombinant protein production and drug discovery applications. simple baked bone in chicken breast recipeWebSequences of PhiC31 and BxB1 attachment sites For each recombinase, the attB and attP attachment sites share a common central dinucleotide sequence (in red) that is used for … rave recliner seatsWebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB … raver dances his shoes offWebAug 20, 2024 · PhiC31 integrase-based vectors can integrate therapeutic genes selectively into attP or pseudo-attP sites in genomes, but considerable numbers of pseudo-attP sites … rave recordsWebFeb 20, 2013 · Introduction. phiC31 integrase is a site-specific recombinase, which is produced by a kind of bacteriophage (phiC31) in a strain of Streptomyces (1, 2).This enzyme is a member of serine recombinases that has an accurate and specific function to recombine DNA fragments between two identified sites, with approximately 30 base pairs … rave religion finch lyrics