Phospho-slp-76 ser376
WebLymphocyte cytosolic protein (SLP-76) is required for T-cell activation and has been implicated in a number of signaling pathways. SLP76 is phosphorylated at Ser376 by HPK1, and this phosphorylation leads to a decrease in T-cell receptor signaling. o Description of the LANCE Ultra Assay WebThe Mouse Total SLP-76 cellular assay kit is designed to monitor the expression level of SLP-76, whether phosphorylated or unphosphorylated. It is compatible with our Mouse phospho-SLP-76 (Ser376) kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readouts of T-lymphocyte activation. Benefits
Phospho-slp-76 ser376
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WebJan 20, 2024 · We report here that SLP76-S376A-expressing mice exhibit normal thymocyte development and no detectable phenotypic alterations in mature T cell subsets or other lymphoid and myeloid cell lineages. Biochemical analyses revealed that mutant T cells were hypersensitive to TCR stimulation. Webtion of SLP-76 at Ser376, but does not affect the level of total SLP-76 protein. The relationship between lysate protein concentration from untreated and H 2 O -treated Jurkat cells and the absorbance at 450 nm using the FastScan™ Phospho-SLP-76 (Ser376) ELISA Kit #30794 is shown in the upper figure. The corresponding western blots using
Webnm using the FastScan™ Phospho-SLP-76 (Ser376) ELISA Kit (Human Preferred) #25424 is shown in the upper figure. The cor-responding western blots using phospho-SLP-76 (Ser376) antibody (left panel) and SLP-76 antibody (right panel) are shown in the lower figure. After serum starvation, Jurkat cells were either left untreated or treated with 11 ... WebSLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T …
Web(14745) Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb Supplier Cell Signaling Technology. Host Rabbit Type Primary Clonality Recombinant (D9D6E) Recombinant Yes Conjugate Unconjugated Target Lymphocyte cytosolic protein 2 (Human) UniProt Q13094 - LCP2_HUMAN. Gene LCP2 Modification WebPhospho-SLP-76 (Ser376) control lysate. This lysate is a component of the phospho-SLP-76 (Ser376) kit. It may be used as a residue specific positive control for SLP-76 phosphorylation. Prices may differ between countries.
Web15 min; green), using Phospho-SLP-76 (Ser376) (E3G9U) XP® Rabbit mAb #76384 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Western blot analysis of extracts from Jurkat cells ...
WebSLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. dutch bros forbesWebSLP-76 phosphorylated at Ser376 and another that recognizes an invariant epitope of SLP-76. ... Eu-Ab1 and FR-Ab2 (5 µL) for detection of phospho-SLP-76 (S376). • The plate was incubated at RT for . 18 hours. and the TR-FRET signal . was recorded at 665 and 615 nm (EnVision®; lamp excitation). cryptoplanes meWebFeb 27, 2024 · Image: ELISA detection of phospho-SLP-76 (Ser376) using a FastScan™ sandwich ELISA kit from Cell Signaling Technology. Treatment efficacy is supported by Western blot analysis. Consider using automation dutch bros fill a trayWebMonoclonal Antibody for studying SLP76 (Ser376) phosphate. Validated for WB, IP, IHC, IHC, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SLP-76 … cryptoplanes marketplaceWebAs Phospho SLP76 (Ser396) is a readout of T cell activation, it was used to monitor the inhibitory effect of PD-L1 recombinant protein on T cell activation. This assay was performed using the two-plate assay protocol. 400 000 Jurkat cells were plated in 25 µL of complete RPMI culture medium with 10% FBS. cryptoplane coin to thbWebSLP-76 (Phospho-Ser376) Rabbit Polyclonal Antibody Product Code AMS.BT-AP04769-100ul Skip to the end of the images gallery Skip to the beginning of the images gallery $230.00 Qty Add to Cart Request Quote for Bulk Pricing Order Support T: +1 (617) 945 5033 T: +1 (800) 987 0985 (toll free) F: +1 (617) 945 8218 Email: [email protected] cryptoplanes thbWebThe Phospho-SLP-76 (Tyr145) assay measures SLP-76 when phosphorylated at Ser376. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Phospho-SLP-76 (Tyr145) assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor. dutch bros financial statements